Heat induction greater than ten minutes results in transformation of parasegments 1 through 5 into five additional copies of parasegment 6 with parasegment 6 like denticle belts. Head involution is completely blocked, formation of head specific structures is suppressed. Formation of both the Keilin's organs and ventral pits is suppresses in all three thoracic parasegments. Very short heat shocks 72 hours after egg laying produce viable adult flies with transformation of antennae into legs, rare transformations of eye into thoracic cuticle causing a rough eye phenotype and few transformations of haltere to wing material.
Carried in a plasmid and expressed in Schneider line 2 cells.
Carried in plasmid "pPhsUBX Ib". Expressed in Drosophila S2 cells.