The mean number of sperm in the seminal vesicles of males expressing Cbβ\DT-AE148D.Acp95EF is not significantly different from that of control males. The males transfer more variable numbers of sperm during mating than control males and the amount of sperm transferred is marginally significantly different from that of control males. The number of sperm stored in both the seminal receptacle and spermathecae of females mated to males expressing Cbβ\DT-AE148D.Acp95EF is significantly less than the number of sperm stored by females mated to control males.
Males seminal fluid contains 'greatly reduced' main-cell products. Female recipients of 'full' main-cell products (tud1) have significantly shorter lifespans than female recipients of 'reduced' (Cbβ\DT-AE148S.Acp95EF) or 'greatly reduced' main-cell products (Cbβ\DT-AE148D.Acp95EF), although they mated significantly less frequently.
Females mated to Cbβ\DT-AE148D.Acp95EF males store significantly fewer sperm on average compared to females mated to control males. Most of the females have some sperm in their ventral receptacle, although less than in controls, and only 17% have no or very few sperm.
Females mated to Cbβ\DT-AE148D.Acp95EF males show defects in the stimulation of egg laying that normally occurs after mating. Approximately 25% of the females lay eggs at an elevated rate comparable to that of females mated to control males, but 20% lay eggs at a non-elevated rate comparable to that of unmated females, and most of the remaining females lay eggs at a partially stimulated rate on days 1 and 2 after mating.