diphtheria toxin, Cbβ\DT-A
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Cbeta\DT using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Cbeta\DT in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
tud males produce seminal fluid but no spermatozoa, transgenic Cbβ\DT-A males produce no detectable spermatozoa and no detectable main-cell products in the seminal fluid. Lifespan of females mated to Cbβ\DT-A males is greater than those mated to tud males (FBrf0079936). The entire cost of mating is attributable to receiving male main-cell products, the main cell products have a quantitative effect (FBrf0079936).
A method that uses Cbβ\DT-A, the Scer\FLP1 recognition target sequences and the Scer\GAL4-Scer\UAS activation system can be used to ablate specific neurons in the embryo and to examine the consequences.
Accessory gland function has been determined by diphtheria toxin directed cell ablation. Only accessory gland secretions are essential for the short-term inhibition to remating. Long term inhibition to remating depends only on the storage of sperm in the female.
Temperature sensitive Cbβ\DT-A mutants are expressed in Drosophila and temperature dependence of the mutant toxin is assayed.
Toxin-mediated ablation is used to study aspects of the visual system development.