Mutant males make no detectable accessory gland proteins in the main cells of their accessory glands (96% of the gland). They make secondary cell. ejaculatory bulb, and ejaculatory duct proteins.
Wild-type females mated to Cbβ\DT-AAcp95EF.PK males begin to deposit eggs only at 10 hours after mating and deposit 5.9 +/- 2 eggs over a 24 hour period, a level not significantly different from that seen for virgin females. The oocyte accumulation pattern in wild-type females mated to Cbβ\DT-AAcp95EF.PK males is similar to that of virgin females, although the number of oocytes at stages 8-9 and 10A-12 in these females is significantly increased compared to those found in virgin females.
Females mated to males expressing Cbβ\DT-AAcp95EF.PK have an autofluorescent mating plug in the right location. However, these mating plugs contain only the fluorescent posterior mating plug and do not contain the anterior non-autofluorescent mating plug.
Wild-type females mated to wild-type males and then remated with males carrying Cbβ\DT-AAcp95EF.PK have the same high egg hatch rate shortly after the remating as females remated with the wild-type males.
Males do not produce sperm or accessory gland main cell secretions. Females mated to these males are courted by males approximately half as vigorously as virgin females when tested 8 or 10 hours after the initial mating. When tested 24 hours after the initial mating, the attractiveness of the females is not significantly different from that of virgin females.
No detectable main-cell products in seminal fluid. They also lack sperm but produce secondary cell, ejaculatory duct and bulb products. Median life span of females receiving main-cell products from tud1 males is shorter than females receiving no main cell products from Cbβ\DT-AAcp95EF.PK males.
Accessory glands of Cbβ\DT-AAcp95EF.PK males are slightly smaller and thinner normal less than 1 hour after eclosion. The glands fail to grow as the flies age, and instead the binucleate main cells become disorganised and eventually degenerate. The males do not produced sperm.
Females mated to Cbβ\DT-AAcp95EF.PK males have a normal-looking mating plug. The egg-laying rate of these mated females is almost identical to that of unmated females. The majority of females are receptive to control males one day after previously mating.
Encodes the full strength Cbβ\DT-A.
Cbβ\DT-A in the main cells kills the cells by blocking protein translation. Microcauterised males are used as the controls for any difference in non-mating exposure to female survival.