FlyBase Allele Report: Scer\GAL4[NPFR.6.6]

General Information

Symbol

Scer\GAL4^NPFR.6.6

Species

S. cerevisiae

Name

FlyBase ID

FBal0152217

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{NPFR-GAL4.6.6}

Also Known As

npfr1-gal4, npfr-gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Wu et al., 2003)

Progenitor genotype

Carried in construct

P{NPFR-GAL4.6.6}

Cytology

Description

A 6.6kb fragment containing NPFR upstream sequence and the first exon regulates expression of a GAL4 driver.

(Wu et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

NPFR

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval ventral nerve cord | restricted

(Wu et al., 2003)

neuron | subset of larval ventral nerve cord

(Wu et al., 2003)

Additional Information

Statement

Reference

Expression is observed in neuropeptide F receptor-positive neurons.

(Wu et al., 2003)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage, with Cbeta\DT^A-I.UAS

(Wu et al., 2003)

abnormal feeding behavior, with NPFR^RNAi.UAS

(Wu et al., 2005)

abnormal feeding behavior, with NPFR^UAS.cYa

(Wu et al., 2005)

abnormal feeding behavior, with Pi3K92E^UAS.Tag:MYC

(Wu et al., 2005)

abnormal feeding behavior | larval stage, with Cbeta\DT^A-I.UAS

(Wu et al., 2003)

abnormal sleep | heat sensitive, with TrpA1^UAS.cUa

(Chung et al., 2017)

chemical resistant, with Cbeta\DT^A-I.UAS

(Wen et al., 2005)

chemical resistant | heat sensitive, with shi^1.UAS

(Wen et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Acute thermogenetic activation (using TrpA1^Scer\UAS.cUa at 29[o]C) of Scer\GAL4^NPFR.6.6 neurons results in a significant decrease of sleep amount (especially during the 12hr dark phases), compared to controls. This short-sleep phenotype progressively declines with prolonged activation, particularly during the light phase.

(Chung et al., 2017)

Inhibition of neural activity in Scer\GAL4^NPFR.6.6-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^NPFR.6.6/Ns3^UAS.Venus fails to rescue Ns3^G0431

(Kaplan et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^NPFR.6.6

Scer\GAL4^NPFR1.6.6

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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