FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lacZen-Xho25
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General Information
Symbol
Ecol\lacZen-Xho25
Species
E. coli
Name
FlyBase ID
FBal0041284
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
P{en-lacZ(Xho)}enXho25
Carried in Construct
Also Known As
en-lacZ, engrailed-lacZ, ryXho25, PlacZ rhx25, enXho25-lacZ
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
p pi25.7wc
(Hama et al., 1990)
P-element activity
(Akimaru et al., 1997, Kopp and Duncan, 1997, Kopp et al., 1997, Struhl et al., 1997)
Progenitor genotype
Ecol\lacZen.Xho
(Hama et al., 1990)
Associated Insertion(s)
P{en-lacZ(Xho)}enXho25
Cytology
Description

Ecol\lacZ gene expression is driven by an en promoter.

(Capdevila et al., 1994)

Ecol\lacZ expressed from an en promoter.

(Heemskerk and DiNardo, 1994)

Expression of Ecol\lacZ driven by en promoter.

(Sampedro et al., 1993)

Construct: Ecol\lacZ gene expression is driven by en regulatory regions.

(Eberl et al., 1992)

Ecol\lacZ expression is under the control of the en promoter.

(Vincent and O'Farrell, 1992, Simcox et al., 1989)

Ecol\lacZ gene expression driven by en promoter region.

(Simcox et al., 1991)

Ecol\lacZ gene under the control of an en promoter.

(Williams et al., 1990)

Ecol\lacZ gene expression is driven by en promoter region.

(Busturia et al., 1989)

Ecol\lacZ gene expression driven by a region of the en promoter.

(Busturia and Morata, 1988)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 9 -- 11
      ventral neurectoderm & embryonic mandibular segment | restricted
      (Urbach et al., 2003)
      deutocerebral neuroblast | subset
      (Urbach and Technau, 2003)
      ventral neurectoderm & embryonic antennal segment | restricted
      (Urbach et al., 2003)
      tritocerebral neuroblast | subset
      (Urbach and Technau, 2003)
      ventral neurectoderm & embryonic head segment | dorsal | restricted
      (Urbach et al., 2003)
      ventral neurectoderm & embryonic labral segment | restricted
      (Urbach et al., 2003)
      protocerebral neuroblast | subset
      (Urbach and Technau, 2003)
      procephalic neuroblast | subset
      (Urbach et al., 2003)
      ventral neurectoderm & embryonic intercalary segment | restricted
      (Urbach et al., 2003)
      third instar larval stage
      wing disc | posterior
      (Guillen et al., 1995)
      haltere disc posterior compartment
      (Tashiro et al., 1993)
      eye disc posterior compartment
      (Tashiro et al., 1993)
      antennal disc posterior compartment
      (Tashiro et al., 1993)
      wing disc posterior compartment
      (Tashiro et al., 1993)
      leg disc posterior compartment
      (Tashiro et al., 1993)
      presumptive ocellus
      (Tashiro et al., 1993)
      larval abdominal segment | posterior
      (Struhl et al., 1997)
      posterior dorsal histoblast nest abdominal
      (Struhl et al., 1997)
      ventral histoblast nest abdominal | posterior | subset
      (Struhl et al., 1997)
      P-stage
      wing posterior compartment
      (Blair, 1992)
      adult stage
      wing posterior compartment
      (Blair, 1992)
      adult abdominal segment posterior compartment
      (Struhl et al., 1997)
      Additional Information
      Statement
      Reference

      Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d3, d5, v4, v5; deuterocerebrum - d5, d9, d13, v8; protocerebrum - ad3-10, av1, cv4, pd5, pd8

      (Urbach and Technau, 2003)

      Expression of en-lacZ reporter delimits the segmental boundaries in the embryonic procephalic region of the ectoderm and labels a subset of neuroblasts during stages 9-11.

      (Urbach et al., 2003)

      In third instar larval abdominal segments, expressed in the posterior compartment, in the posterior dorsal histoblast nest, and in a posterior subset of the cells in the ventral histoblast nests. In adult abdominal segments, expressed in the posterior compartment.

      (Struhl et al., 1997)

      EcollacZen-Xho25 transcript was detected in the posterior compartments of imaginal discs, and in ocellus primordia.

      (Tashiro et al., 1993)

      In pupal wing, pattern slightly different that detected by antibodies against en protein. High level of expression in posterior compartment of the wing; in addition, a narrow region of faintly labelled cells immediately anterior to the posterior compartment, within the anterior compartment and widest at the distal tip of the wing.

      (Blair, 1992)

      Expression used as a marker for the posterior compartments of discs. The exact pattern was reported elsewhere. (FBrf0051823).

      (Simcox et al., 1991)
       
      Marker for
      Dd13 neuroblast
      (Urbach and Technau, 2003)
      Dd5 neuroblast
      (Urbach and Technau, 2003)
      Dd9 neuroblast
      (Urbach and Technau, 2003)
      Dv8 neuroblast
      (Urbach and Technau, 2003)
      Pad13 neuroblast
      (Urbach and Technau, 2003)
      Pad3 neuroblast
      (Urbach and Technau, 2003)
      Pad4 neuroblast
      (Urbach and Technau, 2003)
      Pad5 neuroblast
      (Urbach and Technau, 2003)
      Pad6 neuroblast
      (Urbach and Technau, 2003)
      Pad7 neuroblast
      (Urbach and Technau, 2003)
      Pad8 neuroblast
      (Urbach and Technau, 2003)
      Pad9 neuroblast
      (Urbach and Technau, 2003)
      Pav1 neuroblast
      (Urbach and Technau, 2003)
      Pcv4 neuroblast
      (Urbach and Technau, 2003)
      Ppd8 neuroblast
      (Urbach and Technau, 2003)
      Td3 neuroblast
      (Urbach and Technau, 2003)
      Td5 neuroblast
      (Urbach and Technau, 2003)
      Tv4 neuroblast
      (Urbach and Technau, 2003)
      Tv5 neuroblast
      (Urbach and Technau, 2003)
      imaginal disc posterior compartment
      (Simcox et al., 1991)
      neuroblast DALv2
      (Urbach and Technau, 2003)
      Reflects expression of
      en
      (Urbach and Technau, 2003, Urbach et al., 2003, Kennerdell and Carthew, 2000, Struhl et al., 1997, Guillen et al., 1995, Blair, 1992)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Comments
      Comments

      Ecol\lacZen-Xho25 has been used to show that the mixed clones of cells (marked with a lineage tracer at the blastoderm stage) seen at the posterior edge of the embryonic en stripes are caused by some of the cells losing en expression.

      (Vincent and O'Farrell, 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Ecol\lacZen-Xho25
      Xho25
      (Wilkie et al., 1999)
      enXho25
      (Latham et al., 2013)
      lacZen-Xho25
      rhx25
      (Nassif et al., 1998)
      ryXho25
      (Agrawal et al., 1995)
      Name Synonyms
      Secondary FlyBase IDs
        References (92)