FlyBase Allele Report: Dmel\N[int.Act5C]

General Information

Symbol

Dmel\N^int.Act5C

Species

D. melanogaster

Name

FlyBase ID

FBal0042684

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{Act5C(-FRT)N.intra}

Key Links

Transgenic product class

inframe_deletion

(Struhl et al., 1993)

Mutagen

FLPase

Nature of the Allele

Transgenic product class

inframe_deletion

(Struhl et al., 1993)

Mutagen

FLPase

(Reddy et al., 1997, Larkin et al., 1996, Struhl et al., 1993)

Progenitor genotype

N^{int.FRT.Act5C}

(Reddy et al., 1997, Larkin et al., 1996, Struhl et al., 1993)

Carried in construct

P{Act5C(-FRT)N.intra}

Cytology

Description

The FRT cassette containing y[+] has been excised, allowing expression of the activated N receptor under the control of the Act5C promoter.

(Reddy et al., 1997)

FRT cassette has been excised, juxtaposing Act5C promoter with N coding sequences.

(Struhl et al., 1993)

Allele components

Component

Use(s)

Regulatory region(s)

Act5C

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antennal sense organ | somatic clone

(Reddy et al., 1997)

female germline stem cell | increased number

(Ward et al., 2006)

germarium cap cell | increased number

(Ward et al., 2006)

neuroblast

(Struhl et al., 1993)

stalk follicle cell

(Larkin et al., 1996)

Detailed Description

Statement

Reference

Expression of N^int.Act5C in the female germline results in germaria with extra cap cells and additional germ-line stem cells.

(Ward et al., 2006)

Homozygous clones resemble N null clones and show a failure of sensilla development.

(Reddy et al., 1997)

Generates long stalk-like structures in the ovaries, stalks are thicker than wild type stalks. The severity of the stalk phenotype correlates with the length of the precursor cell stage, culminating in a complete block at the precursor stage.

(Larkin et al., 1996)

In embryos and in imaginal discs, neuroblast segregations are suppressed and all ectodermal cells give rise to epidermis.

(Struhl et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Exists in clones, not reported as being germ-line transmitted. The product of this allele becomes nuclear-localized. Behaves as a gain of function mutation, even in the absence of endogenous N activity, and has its effect when cell fate decisions are being made.

(Struhl et al., 1993)

Exists in clones, not reported as being germ-line transmitted.

(Reddy et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

N^Act5C.PS

N^int.Act5C

caN-intermed

(Larkin et al., 1996)

caN-strong

(Larkin et al., 1996)

Name Synonyms

constitutively active N-intermediate

(Larkin et al., 1996)

constitutively active N-strong

(Larkin et al., 1996)

Secondary FlyBase IDs

References (4)

Report Sections

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