Point mutation in the δ and γ fkh binding sites.
Construct: Complete transcription unit of Sgs4, plus 2490bp of the upstream sequence and approximately 3800bp of downstream sequence. EcR element I is deleted and base pair substitutions have been generated in element II.
Used to assay level of expression of Sgs4 gene in an Sgs4K background, relative to that of Sgs3 : expression dramatically reduced.