Point mutation in the δ fkh binding site.
Construct: Complete transcription unit of Sgs4, plus 2490bp of the upstream sequence and approximately 3800bp of downstream sequence. Base pair substitutions have been generated in EcR element II.
Used to assay level of expression of Sgs4 gene in an Sgs4K background, relative to that of Sgs3 : expression reduced.
Used to assay level of expression of Sgs4 gene in an Sgs4K background, relative to that of Sgs3 : expression reduced.