5.7kb BamHI fragment from position -5 to +0.7 (coordinates based on the map of FBrf0040194), is fused upstream of Ecol\lacZUbx.P140.
6kb fragment of Ubx regulatory sequences, from position -5.5 to +0.5 (coordinates according to the map of FBrf0040194), drives the expression of Ecol\lacZUbx.P140.