200bp SmaI PstI fragment that spans from -5.4kb to -5.2kb within the eve autoregulatory element drives lacZ expression.
200bp SmaI PstI fragment of eve promoter sequence (located within the 1.6kb XbaI KpnI fragment from position -6.3kb to -4.7kb) is fused upstream of a minimal Hsp70 promoter followed by the lacZ coding sequence.
Fragment of the eve promoter includes the embryo nuclear factors 1 to 3 (DENF1, DENF2 and DENF3) and the two eve-binding sites (E-D and E-P).