FlyBase Allele Report: Dmel\en[UAS.cTa]

General Information

Symbol

Dmel\en^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Tabata

FlyBase ID

FBal0045601

Feature type

allele

Associated gene

Dmel\en

Associated Insertion(s)

Carried in Construct

P{UAS-en.T}

Also Known As

UAS-en

Key Links

Transgenic product class

cDNA

(Tabata et al., 1995)

wild_type

(Tabata et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Tabata et al., 1995)

wild_type

(Tabata et al., 1995)

Mutagen

in vitro construct

(Tabata et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-en.T}

Cytology

Description

A EcoRI cDNA fragment containing the en ORF is expressed from the UASt regulatory sequences.

(Tabata et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{171aa.138Q.UAS.Tag:HA}

(Mugat et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal row of triple row of wing sensilla, with Scer\GAL4^112-97

(Tabata et al., 1995)

dorsal row of triple row of wing sensilla, with Scer\GAL4^en-e16E

(Tabata et al., 1995)

embryonic/first instar larval cuticle, with Scer\GAL4^prd.RG1

(Kobayashi et al., 2003)

embryonic hindgut, with Scer\GAL4^byn-Gal4

(Takashima and Murakami, 2001)

haltere, with Scer\GAL4^112-97

(Tabata et al., 1995)

medial row of triple row of wing sensilla, with Scer\GAL4^112-97

(Tabata et al., 1995)

medial row of triple row of wing sensilla, with Scer\GAL4^en-e16E

(Tabata et al., 1995)

ventral row of triple row of wing sensilla, with Scer\GAL4^112-97

(Tabata et al., 1995)

ventral row of triple row of wing sensilla, with Scer\GAL4^en-e16E

(Tabata et al., 1995)

wing, with Scer\GAL4^112-97

(Tabata et al., 1995)

wing blade posterior compartment

(Tabata et al., 1995)

wing vein, with Scer\GAL4^112-97

(Tabata et al., 1995)

wing vein, with Scer\GAL4^en-e16E

(Tabata et al., 1995)

Detailed Description

Statement

Reference

Expression of en^Scer\UAS.cTa in even segments (but not odd) of the developing embryo, under the control of Scer\GAL4^prd.RG1 leads to the fusion of anterior and posterior commissures or to the absence altogether of these commissures. In the latter case, the longitudinal tracts appear thicker. The number of neuronal cells remains unchanged in these embryos, indicated a misrouting phenotype rather than apoptosis.

Expression of en^Scer\UAS.cTa under the control of Scer\GAL4^da.G32, a ubiquitous early driver, leads to axonal pathfinding defects.

Expression of en^Scer\UAS.cTa under the control of Scer\GAL4^sca.PC, which drives expression in the neuroblasts and neurons, leads to axonal pathfinding defects.

Expression of en^Scer\UAS.cTa under the control of pan-neuronal drivers such as Scer\GAL4¹⁴⁰⁷ or Scer\GAL4^elav.PLu does not generate any major defect in the overall architecture of the ventral nerve cord. At most, some low penetrance effects (less than 1%) are observed.

(Joly et al., 2007)

en^Scer\UAS.cTa; Scer\GAL4^prd.RG1 embryos have severe cuticle defects.

(Kobayashi et al., 2003)

When en^Scer\UAS.cTa is expressed under the control of Scer\GAL4^byn-Gal4, the border cells of the hindgut (which normally form an anterior and posterior ring at the ends of the hindgut and bilateral strands that connect the two rings) fail to form, except at the posterior border of the large intestine.

(Takashima and Murakami, 2001)

When expressed under the influence of Scer\GAL4^112-97, abnormal wings and halteres develop. Some of the medial triple row bristles along the anterior wing margin are transformed to posterior type, anterior wing shows outgrowths and venation is abnormal. Anterior effects are similar to those caused by In(2R)vg^W. Rare partial transformations of haltere to wing (similar to those caused by In(2R)vg^W) occur. When expression is driven by Scer\GAL4^en-e16E, surviving flies have wings with normal patterns of veins and bristles in the anterior compartment, but abnormal veins and triple row bristles in the posterior compartment. Some wings had outgrowths while others had anterior transformations that were not accompanied by outgrowths.

(Tabata et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^prd.RG1, en^UAS.cTa has embryonic/first instar larval cuticle phenotype, suppressible | partially by exd¹

(Kobayashi et al., 2003)

Other

Statement

Reference

N^ΔE.UAS, Scer\GAL4^byn-Gal4, en^UAS.cTa has embryonic hindgut phenotype

(Takashima et al., 2002)

N^ΔE.UAS, Scer\GAL4^byn-Gal4, en^UAS.cTa has embryonic large intestine phenotype

(Takashima et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of cuticle defects seen in en^Scer\UAS.cTa; Scer\GAL4^prd.RG1 embryos is reduced in embryos maternally exd¹/exd¹ and zygotically exd¹/+.

(Kobayashi et al., 2003)

When Dl::N^{ΔECN.Scer\UAS} and en^Scer\UAS.cTa are co-expressed under the control of Scer\GAL4^byn-Gal4, the entire large intestine differentiates into boundary cells.

(Takashima et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

en^Scer\UAS.cTa

en^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Tabata

(Tabata et al., 1995)

Secondary FlyBase IDs

References (13)

Report Sections

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