Construct: 2.3kb Asp718-SalI fs(1)K10 fragment (nucleotides 1-3112 including the nurse cell enhancer/promoter, the entire protein coding region and the first 65 nucleotides of the 3' UTR) fused to DraI-PstI fragment containing the last 133 nucleotides of the 3' UTR.
Different lines of P{K10.Δ3'} vary in their ability to restore female fertility to fs(1)K10LM00 mutants.