FlyBase Allele Report: Avic\GFP[Ubi-p63E.Tag:NLS(Unk)]

General Information

Symbol

Avic\GFP^{Ubi-p63E.Tag:NLS(Unk)}

Species

A. victoria

Name

FlyBase ID

FBal0047086

Feature type

allele

Associated gene

Avic\GFP

Associated Insertion(s)

Carried in Construct

P{Ubi-GFP.nls}

Also Known As

ubi-nlsGFP, nlsGFP, Ubi-GFPnls, NLS-GFP, ubi-nls GFP

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Davis et al., 1995)

Progenitor genotype

Carried in construct

P{Ubi-GFP.nls}

Cytology

Description

1980bp of Ubi-p63E promoter (and first four amino acids) directs the expression of a 9 amino acid nuclear localization sequence fused to 40 amino acids of Ecol\lacZ (from its N-terminus) fused to full length GFP followed by Hsp27 polyadenylation sequence.

(Davis et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Ubi-p63E

Encoded product / tool

GFP

green fluorescent protein

Tagged with

Tag:NLS(Unk)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Davis et al., 1995)

Additional Information

Statement

Reference

In early embryo, used as nuclear marker for identification of cells within mitotic domains.

(Namba and Minden, 1999)

Shows highly fluorescent green nuclei and less fluorescent cytoplasm. (Fusion protein contains nuclear localization signal.) There is a long delay between zygotic expression and appearance of fluorescence.

(Davis et al., 1995)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease