Contains βTub85D sequence from -53bp to +156bp fused to lacZ. Also has an artificial BglII site between -38bp and -33bp of the βTub85D upstream sequence, created by the following nucleotide substitutions G-34C, T-36A and T-37G.
TheEcollacZβTub85D.Mut1 transcript recapitulates wild type βTub85Dexpression, and therefore contains all sequences necessary to drive testesspecific expression.