lacZ gene expression is driven by 700bp rho promoter fragment that lacks native sna repressor sites, but has two Kr binding sites. Most proximal Kr binding site is positioned 180bp from start of lacZ transcription and 55bp from nearest dl binding site.
Used as part of an analysis of the function of CtBP as a repressor in regulating gene expression in the patterning the early embryo.
Used to investigate short range transcriptional repression in this synthetic modular promoter.