FB2026_02 , released June 18, 2026
Allele: Dmel\trhDN.UAS.Tag:T7
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General Information
Symbol
Dmel\trhDN.UAS.Tag:T7
Species
D. melanogaster
Name
Dominant Negative
FlyBase ID
FBal0050668
Feature type
allele
Associated gene
Dmel\trh
Associated Insertion(s)
Carried in Construct
P{UAS-trh.DN}
Key Links
Transgenic product class
inframe_deletion
(Wilk et al., 1996)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Wilk et al., 1996)
Mutagen
in vitro construct
(Wilk et al., 1996)
Progenitor genotype
trhUAS.cWa
(Wilk et al., 1996)
Carried in construct
P{UAS-trh.DN}
Cytology
Description

Deletion of the transcription activation domain of the trh coding region. Resulting sequence is expressed from the UASt regulatory sequences, and tagged with a Tag:T7 tag.

(Wilk et al., 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
trh
Tagged with
Tag:T7
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When expression is driven by Scer\GAL4hs.2sev heat shocked 2.5hr after egg laying, tracheal development in the embryo is abnormal, no lumen appears. Invaginations of a few tracheal cells that express a small patch of lumen material are occasionally observed. Effects are only seen with two copies of P{UAS-trh.DN}. Induction 4hr AEL does not perturb the development of the tracheal pits, though cells in the dorsal trunk in each segment fail to fuse with their neighbours.

      (Wilk et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      trhDN.Scer\UAS.T:Uuuu\T7
      trhDN.UAS.T:T7
      trhDN.UAS.Tag:T7
      Name Synonyms
      Dominant Negative
      (Wilk et al., 1996)
      Secondary FlyBase IDs
        References (1)