Construct: Amino acid replacement: S?Q. Amino acid replacement: N?Q. S?Q and N?Q fall at positions 9 and 10 of the homeodomain, respectively. The mutant prd protein is expressed from a heat shock promoter.
Carried in plasmid and assayed in Schneider cells for ability to activate transcription from "p3K'-TATA-CAT" and "pPB3-CAT".
Carried in plasmid and assayed in Schneider cells for ability to activate transcription from "p3K'-TATA-CAT" and "pPB3-CAT".