Construct: The P-element second intron is inserted into the I-element\RTase coding region.
The I-element carrying I-element\RTaseπI is unable to transpose in the absence of other I-elements, due to the insertion of a P-element intron into the coding region. The P-element intron is removed during transposition of the I-element carrying I-element\RTaseπI, indicating that the I-element transposes via an RNA intermediate.
The I-element carrying I-element\RTaseπI is unable to transpose in the absence of other I-elements, due to the insertion of a P-element intron into the coding region. The P-element intron is removed during transposition of the I-element carrying I-element\RTaseπI, indicating that the I-element transposes via an RNA intermediate.