550bp HindIII-PstI fragment derived from Hame\AFP cDNA clone C2-1, coding for exons 1-4, is fused to genomic Hame\AFP DNA coding for intron 4 and exon 5. Expression is driven by 370bp BglI-NsiI promoter fragment from the Yp1-Yp2 intergenic region.
Comments on Models/Modifiers Based on Experimental Evidence
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Disease-implicated variant(s)
Phenotypic Data
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When compared to haemolymph from nontransgenic flies P{YP1:srAFP} haemolymph formed smaller ice crystals at -10oC, -3oC and at the onset of melting, indicating inhibition of ice recrystallization.
Female transgenic flies bearing the construct synthesize Hame\AFP and secrete it into their haemolymph, male transgenics produce no Hame\AFP and serve as controls.
Female transgenic flies bearing the construct synthesize Hame\AFP and secrete it into their haemolymph, male transgenics produce no Hame\AFP and serve as controls.