Acp36DE2/Df(2L)H20 males show the same success as control Acp36DE+ males in achieving fertilisations when they are the second males to mate with females. Acp36DE2/Df(2L)H20 males achieve a wild-type number of fertilisations as first-mating males when females are first mated to Acp36DE2/Df(2L)H20 males and then to wild-type males.
Sperm from mutant males show significantly altered resistance to displacement from the female's reproductive tract by subsequent sperm.