The anterior mating plug does not form in females mated to Acp36DE1 males.
Acp36DE1/Df(2L)H20 males are significantly less successful than control Acp36DE+ males in achieving fertilisations when they are the second males to mate with females. Acp36DE1/Df(2L)H20 males which lack sperm (due to irradiation) do not differ from spermless control males in their ability to displace resident first-male sperm when they are the second males to mate with females. Acp36DE1/Df(2L)H20 males achieve significantly fewer fertilisations as first-mating males compared with control Acp36DE+ males when females are first mated to Acp36DE1/Df(2L)H20 males and then to wild-type males. In the first two days after remating, females first mated to spermless (due to irradiation) Acp36DE1/Df(2L)H20 males and then wild-type males, produce significantly fewer second-mlae progeny than females first mated to spermless Acp36DE+ males.
Sperm from mutant males show significantly altered resistance to displacement from the female's reproductive tract by subsequent sperm.