Construct: 1.1kb BamHI-SalI genomic RpLP2 fragment including promoter and 5' transcribed sequences is fused in frame to a 4.0kb SalI-KpnI ftz genomic fragment which encodes the 3' two thirds of the transcribed sequences.
Poly(A)-containing RNAs prepared from synchronous embryos are analysed on RNA blots to measure mRNA stability.
Poly(A)-containing RNAs prepared from synchronous embryos are analysed on RNA blots to measure mRNA stability.