Expression of a 2kb EcoRI cDNA fragment of aos including the complete coding region is driven by a Hsp70 promoter.

aos^hs.PSb rescues aos^p1e20

The low viability of argos^p1e20 homozygotes is rescued by two copies of argos^hs.PSb in the absence of heat shock. Expression of argos^hs.PSb using heat shock almost completely rescues the external morphology of the eyes of argos^p1e20 homozygotes. When argos^hs.PSb is expressed using heat pulses after the wandering larval stage in argos^p1e20 homozygotes, only the anterior half of the eye shows normal morphology. Expression of argos^hs.PSb using heat shock also rescues the ommatidial pattern of argos^p1e20 homozygotes. Expression of argos^hs.PSb using a single heat pulse for 1 hour at 38^oC during the third larval instar in argos^p1e20 homozygotes generates a region of the eye in which most ommatidia contain the normal number of 4 cone cells, although some ommatidia in this region contain fewer cone cells than normal. As the distance from the rescued region increases, the number of extra cone cells in the ommatidia gradually increases. The abnormal wing vein and maxillary palp morphology of argos^p1e20 homozygotes is rescued by expression of two copies of argos^hs.PSb using heat shock.