NotI-XbaI fragment containing the entire coding region without either the 5' regulatory regions (between position -755 to +12) or the 3' non-coding regions (between positions +5765 and +5947) is expressed under the control of a heat inducible Hsp70 promoter.
Heat induced expression has no effect on the embryo hatching rate.
The rate of hatching and overall morphology is similar to wild-type in embryos carrying Cg25Ccol.hs, both with or without heat shock.