FlyBase Allele Report: Dmel\rod[X-5]

General Information

Symbol

Dmel\rod^X-5

Species

D. melanogaster

Name

FlyBase ID

FBal0060909

Feature type

allele

Associated gene

Dmel\rod

Associated Insertion(s)

Carried in Construct

Also Known As

rod^X5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Scaerou et al., 1999)

Progenitor genotype

Cytology

Description

Deletion within the rod transcription unit, several changes are detected within the 500bp sequenced - a deletion of 12bp and another of 189bp and substitution of 3 new nucleotides at the end of this deletion, all these changes retain the open reading frame.

(Scaerou et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:31,583,579..31,583,590 [+]

Comment:

One of three lesions associated with this mutation; 12 bp deletion.

(Scaerou et al., 1999)

deletion

3R:31,583,810..31,583,998 [+]

Comment:

One of three lesions associated with this mutation; 189 bp deletion.

(Scaerou et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Scaerou et al., 2001)

Phenotype Manifest In

mitotic anaphase

(Scaerou et al., 2001)

nuclear chromosome

(Scaerou et al., 2001)

Detailed Description

Statement

Reference

Mutant 3rd instar larval brains have a mitotic phenotype; 39.4% of anaphases are abnormal (showing lagging chromatids, chromatin bridges and nondisjunction) in untreated brains. Colchicine-treated mutant brains show 38.2% premature sister chromatid separation (compared to 0.96% in wild type) and have a mitotic index of 0.66 (compared to 2.44 in wild-type treated brains).

(Scaerou et al., 2001)

The mitotic index of mutant larval brains is 0.9, compared to 1.02 in wild-type brains. Mutant brains incubated with colchicine do not show an increase in mitotic index, even after 1 hour of drug treatment, in contrast to wild-type brains, which show a doubling of the mitotic index in this time. Mutant larval brain neuroblasts treated with colchicine often do not arrest in prometaphase (which occurs in wild-type neuroblasts in response to colchicine), but instead enter an anaphase-like state with separated sister chromatids.

(Basto et al., 2000)

Strong allele.

(Williams and Goldberg, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

rod^X-5 has abnormal mitotic cell cycle phenotype, non-enhanceable by Zw10¹⁶

(Scaerou et al., 2001)

NOT suppressed by

Statement

Reference

rod^X-5 has abnormal mitotic cell cycle phenotype, non-suppressible by Zw10¹⁶

(Scaerou et al., 2001)

NOT Enhancer of

Statement

Reference

rod^X-5 is a non-enhancer of abnormal mitotic cell cycle phenotype of Zw10¹⁶

(Scaerou et al., 2001)

Suppressor of

Statement

Reference

rod^X-5 is a suppressor of abnormal mitotic cell cycle phenotype of asp^E3

(Basto et al., 2000)

NOT Suppressor of

Statement

Reference

rod^X-5 is a non-suppressor of abnormal mitotic cell cycle phenotype of Zw10¹⁶

(Scaerou et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

rod^X-5 has nuclear chromosome phenotype, non-enhanceable by Zw10¹⁶

(Scaerou et al., 2001)

rod^X-5 has mitotic anaphase phenotype, non-enhanceable by Zw10¹⁶

(Scaerou et al., 2001)

NOT suppressed by

Statement

Reference

rod^X-5 has nuclear chromosome phenotype, non-suppressible by Zw10¹⁶

(Scaerou et al., 2001)

rod^X-5 has mitotic anaphase phenotype, non-suppressible by Zw10¹⁶

(Scaerou et al., 2001)

NOT Enhancer of

Statement

Reference

rod^X-5 is a non-enhancer of nuclear chromosome phenotype of Zw10¹⁶

(Scaerou et al., 2001)

rod^X-5 is a non-enhancer of mitotic anaphase phenotype of Zw10¹⁶

(Scaerou et al., 2001)

NOT Suppressor of

Statement

Reference

rod^X-5 is a non-suppressor of nuclear chromosome phenotype of Zw10¹⁶

(Scaerou et al., 2001)

rod^X-5 is a non-suppressor of mitotic anaphase phenotype of Zw10¹⁶

(Scaerou et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Abnormal anaphases and premature sister chromatid separation occur at the same frequency in mit(1)15¹⁶ rod^X-5 double mutants as in either single mutant alone. There is no additive effect; the range of defects is quantitatively and qualitatively comparable with that for either single mutant.

(Scaerou et al., 2001)

The mitotic index in asp^E3 rod^X-5 double mutants is reduced compared to asp^E3 single mutants, there is no metaphase arrest and anaphase figures can be seen.

(Basto et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rod^X-5 is rescued by rod^+tC3L9

(Scaerou et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

X-5

(Williams and Goldberg, 1994)

rod^X-5

rod^X5

(Wainman et al., 2009, Williams et al., 2003, Scaerou et al., 2001, Basto et al., 2000, Scaerou et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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