FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ncd2.GFP(S65T)
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General Information
Symbol
Dmel\ncd2.GFP(S65T)
Species
D. melanogaster
Name
FlyBase ID
FBal0061013
Feature type
allele
Associated gene
Dmel\ncd
Associated Insertion(s)
Carried in Construct
P{ncd2-gfp*}
Key Links
Transgenic product class
missense_variant
(Endow and Komma, 1997)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Endow and Komma, 1997)
Mutagen
in vitro construct
(Endow and Komma, 1997, Endow and Komma, 1996)
Progenitor genotype
ncdGFP(S65T)
(Endow and Komma, 1997)
ncd2
(Endow and Komma, 1996)
Carried in construct
P{ncd2-gfp*}
Cytology
Description

An ncd promoter fragment (3.1-kb BamHI-EcoRI fragment containing the promoter and 5' region) drives expression of full-length ncd (amino acids 1 to 700) tagged at the C-terminal end with GFP(S65T). The ncd sequence contains the G446R mutation found in the ncd2 allele (it also contains a D699L amino acid replacement generated during the cloning process).

(Endow and Komma, 1997)
Allele components
Component
Use(s)
Regulatory region(s)
ncd
Encoded product / tool
ncd
Tagged with
GFP(S65T)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of ncd2.T:Avic\GFP-S65T produces abnormal spindles in oocytes. Separation of the spindles at the poles is seen and the spindles fail to elongate into the normal meiosis I spindles typical of wild-type oocytes. Normal meiosis II spindles fail to form and a short multipolar spindle is formed.

      (Endow and Komma, 1997)

      Expression causes spindle abnormalities and mis-segregation of mitotic chromosomes. Free centrosomes arise that exhibit precocious splitting of the centrosome.

      (Endow and Komma, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      ncd2.GFP(S65T) partially rescues ncd1

      (Endow and Komma, 1997)

      ncd2.GFP(S65T) partially rescues ncd2

      (Endow and Komma, 1997)
      Fails to rescue

      ncd2.GFP(S65T) fails to rescue ncd1

      (Endow and Komma, 1996)
      Comments

      ncd2/ncd2 females carrying two copies of ncd2.T:Avic\GFP-S65T have poor fertility, high egg inviability and elevated chromosome missegregation. ncd2/+ females carrying one copy of ncd2.T:Avic\GFP-S65T are completely rescued for chromosome segregation but partially rescued for embryo viability.

      (Endow and Komma, 1997)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ncd2.GFP(S65T)
      ncd2.T:Avic\GFP-S65T
      ncd2.T:GFP-S65T
      Name Synonyms
      Secondary FlyBase IDs
        References (2)