FB2026_03 , released September 17, 2026
Allele: Ecol\lacZGerm.80
Open Close
General Information
Symbol
Ecol\lacZGerm.80
Species
E. coli
Name
FlyBase ID
FBal0061144
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{Germ80Z}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Serano et al., 1994)
Progenitor genotype
Carried in construct
P{Germ80Z}
Cytology
Description

A nurse cell specific regulon, consisting of two copies of the Hsp26 nurse cell enhancer fragment (-543 to -373bp, coordinates according to FBrf0055875) linked to the Sgs3 promoter (-127 to +27bp, coordinates according to FBrf0039858), drives expression of a lacZ coding region fragment (which lacks the first six amino acids). The 3' end of the fs(1)K10 gene (+3956 to +5345bp, coordinates according to FBrf0048127) is fused downstream of the lacZ coding region.

(Serano et al., 1994)
Allele components
Component
Use(s)
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stageoogenesis stage S6 -- S11
      nurse cell
      (Serano et al., 1994)
      adult stageoogenesis stage S6 -- S14
      oocyte
      (Serano et al., 1994)
      Additional Information
      Statement
      Reference

      In germarium, β-gal signal higher in nurse cells than in oocyte until stage S11, at which time the nurse cells donate their entire cytoplasm to the oocyte.

      (Serano et al., 1994)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZGerm.80
      lacZGerm.80
      lacZfs(1)K10.Hsp26.80
      Name Synonyms
      Secondary FlyBase IDs
        References (1)