FB2026_03 , released September 17, 2026
Allele: Dmel\DhfrMtx
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General Information
Symbol
Dmel\DhfrMtx
Species
D. melanogaster
Name
Methotrexate
FlyBase ID
FBal0083556
Feature type
allele
Associated gene
Dmel\Dhfr
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
spontaneous
(Hao et al., 1996)
Progenitor genotype
Cytology
Description

Amino acid replacement: L30Q. Nucleotide substitution: T89A.

(Hao et al., 1996)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:16,481,399..16,481,399 [+]
Nucleotide change:

T16481399A

Reported nucleotide change:

T89A

Amino acid change:

L30Q | Dhfr-PA; L30Q | Dhfr-PB

Reported amino acid change:

L30Q

(Hao et al., 1996)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Selected in S3 cells by resistance to methotrexate. The purified enzyme shows reduced affinity to methotrexate and to trimethoprim, both antifolate drugs.

      (Hao et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DhfrMtx
      Name Synonyms
      Methotrexate
      (Hao et al., 1996)
      Secondary FlyBase IDs
        References (1)