FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\UbxUAS.CD.Tag:HA
Open Close
General Information
Symbol
Dmel\UbxUAS.CD.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0085911
Feature type
allele
Associated gene
Dmel\Ubx
Associated Insertion(s)
Carried in Construct
P{UAS-Ubx.T:CD-1}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Jarvik et al., 1996)
Progenitor genotype
Carried in construct
P{UAS-Ubx.T:CD-1}
Cytology
Description

A CD-cassette (291bp sequence consisting of the 3' end of intron 2 of the α1 tubulin gene of C.reinhardtii (nucleotides 46 to 116) which contains splice branch and acceptor sites, an open reading frame (nucleotides 118 to 172, the 'guest exon') and sequences derived from intron 2 of the α2 tubulin gene of C.reinhardtii (nucleotides 173 to 241) which contain a splice donor site) have been inserted into Ubx intron 3. Expression is driven by UAS regulatory sequences and the open reading frame includes the Tag:HA tag. The result is two chimeric introns surrounding the new 'guest exon'. When Ubx is transcribed, spliced and translated, a protein containing a 'guest peptide' insert is produced.

(Jarvik et al., 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Ubx
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pUMG-101-CD1", cotransfected into SL2 cells with a Scer\GAL4 expression plasmid and assayed for expression of the T:Ivir\HA1 tag present in T:Crei\CD-1T:Ivir\HA1.

      (Jarvik et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      UbxScer\UAS.CD.T:Ivir\HA1
      UbxScer\UAS.T:Crei\CD-1-T:Ivir\HA1
      UbxUAS.CD.Tag:HA
      UbxUAS.T:CD-1-T:HA1
      Name Synonyms
      Secondary FlyBase IDs
        References (1)