FB2026_02 , released June 18, 2026
Allele: Ecol\lacZtetO.cBa
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General Information
Symbol
Ecol\lacZtetO.cBa
Species
E. coli
Name
Tn10 tetracycline operator construct a of Bello
FlyBase ID
FBal0091295
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{tetO.7-lacZ.B}
Also Known As
tetO-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Bello et al., 1998, Girard et al., 1998)
Progenitor genotype
Carried in construct
P{tetO.7-lacZ.B}
Cytology
Description

A heptameric repeat of the tetracycline operator, isolated as a 310bp EcoTI-KpnI fragment, followed by the P\T promoter (up to -42 from the cap site) and N-terminal P\T sequence fused to lacZ.

(Bello et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
tetO
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Used in the tetracyclin (tet)-regulated transactivation system. Addition of tetracycline to the food completely silences Tn10\tetRey.3.5.T:Hsim\VP16-mediated expression of Ecol\lacZTn10\tetO.cBa.

      (Girard et al., 1998)

      Addition of tetracycline to the food completely silences Tn10\tetR-T:Hsim\VP16-mediated expression.

      (Bello et al., 1998)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (10)
      Reported As
      Symbol Synonym
      Ecol\lacZTn10\tetO.cBa
      Ecol\lacZTn10\tetR.O.7
      Ecol\lacZTn10\tetR.O
      Ecol\lacZpSC101\tetR.O.7
      Ecol\lacZpSC101\tetR.O
      Ecol\lacZtetO.cBa
      lacZtetR.O.7
      lacZtetR.O
      tetO-lacZ
      (Bello et al., 1998)
      Name Synonyms
      Tn10 tetracycline operator construct a of Bello
      (Bello et al., 1998)
      Secondary FlyBase IDs
      • FBal0088832
      References (5)