Construct: The stripe 2/UAS sequence from Ecol\lacZeve.ΔH3.2xScer\GAL4 was used to replace the stripe 2 enhancer of Ecol\lacZeve.P2, creating Ecol\lacZ gene whose expression is governed by a stripe 2 enhancer separated from the stripe 3 enhancer by 2 Scer\UAS regulatory sequences and 750 bp of spacer DNA from Avic\GFP.
Used as part of an analysis of the function of CtBP as a repressor in regulating gene expression in the patterning the early embryo.