Deletion that shortens the GAGA repeats from 1kb to approximately 300bp. The first 178 codons of pug (i.e. all that there are in the pugD fusion gene) remain.
Position effect has been eliminated as a possible explanation for lack of phenotype by targeting the construct to different chromosomal locations at which the pugD phenotype has been reproduced using pugK-B.3.4.
Position effect has been eliminated as a possible explanation for lack of phenotype by targeting the construct to different chromosomal locations at which the pugD phenotype has been reproduced using pugK-B.3.4.