FlyBase Allele Report: Dmel\Nos[hs.PK]

General Information

Symbol

Dmel\Nos^hs.PK

Species

D. melanogaster

Name

heat shock construct of Kuzin

FlyBase ID

FBal0105136

Feature type

allele

Associated gene

Dmel\Nos

Associated Insertion(s)

Carried in Construct

P{hs-Nos}

Key Links

Transgenic product class

cDNA

(Kuzin et al., 2000)

wild_type

(Kuzin et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kuzin et al., 2000)

wild_type

(Kuzin et al., 2000)

Mutagen

in vitro construct

(Kuzin et al., 2000)

Progenitor genotype

Carried in construct

P{hs-Nos}

Cytology

Description

Hsp70 promoter sequences drive expression of Nos cDNA.

(Kuzin et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Nos

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage

(Kuzin et al., 2000)

Phenotype Manifest In

eye disc | larval stage

(Kuzin et al., 2000)

Detailed Description

Statement

Reference

Nos^hs.PK flies have no obvious phenotype in the eye. Though the number of cells in S phase in the eye imaginal disc is decreased.

(Kuzin et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nos^hs.PK is a suppressor of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Kuzin et al., 2000)

Other

Statement

Reference

Nos^hs.PK, Rbf^GMR.PD has visible phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has decreased cell number | adult stage phenotype

(Kuzin et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nos^hs.PK is a suppressor of eye phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Kuzin et al., 2000)

Other

Statement

Reference

Nos^hs.PK, Rbf^GMR.PD has pigment cell phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has eye phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has ommatidium phenotype

(Kuzin et al., 2000)

Nos^hs.PK, Rbf^GMR.PD has interommatidial bristle phenotype

(Kuzin et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Rbf^GMR.PD, Nos^hs.PK flies, treated with heatshock before pupariation, lead to adults with multiple defects in the eye, including missing bristles and pigment cells. A lack of the regular number of pigment cells in these flies results in the appearance of many fused ommatidia and a rough eye phenotype. These phenotypes are seen in flies with 2 copies of the transposon responsible for Rbf^GMR.PD (P{GMR-Rbf}) and become more severe when 4 copies of P{GMR-Rbf} are present.

(Kuzin et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nos^hs.PK

Name Synonyms

heat shock construct of Kuzin

(Kuzin et al., 2000)

Secondary FlyBase IDs

References (1)

Report Sections

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