FlyBase Allele Report: Dmel\p53[UAS.cJa]

General Information

Symbol

Dmel\p53^UAS.cJa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Jin

FlyBase ID

FBal0117413

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{UAS-p53.J}

Key Links

Transgenic product class

wild_type

(Jin et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jin et al., 2000)

Mutagen

in vitro construct

(Jin et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-p53.J}

Cytology

Description

The p53 coding region is driven under the control of UASt sequences.

(Jin et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Mendes et al., 2009)

increased cell death, with Scer\GAL4^GMR.PF

(Jin et al., 2000)

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(Bodai et al., 2007)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Jin et al., 2000)

outer photoreceptor cell

(Mendes et al., 2009)

Detailed Description

Statement

Reference

Expression of p53^Scer\UAS.cJa under the control of Scer\GAL4^ninaE.PT induces outer photoreceptor cell death, but not inner photoreceptor cell death (i.e. is cell-autonomous).

(Mendes et al., 2009)

36% of pupariated animals expressing p53^Scer\UAS.cJa under the control of Scer\GAL4^Act5C.PI eclose.

(Bodai et al., 2007)

When p53^Scer\UAS.cJa is ectopically expressed under the control of Scer\GAL4^GMR.PF, a rough eye phenotype can be seen. In the developing retina increased apoptosis is seen.

(Jin et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PI, p53^UAS.cJa has partially lethal - majority die phenotype, enhanceable by Daxx^U26/Daxx^U26

(Bodai et al., 2007)

NOT Enhanced by

Statement

Reference

BacA\p35^GMR.PH, Scer\GAL4^ninaE.PT, p53^UAS.cJa has decreased cell death phenotype, non-enhanceable by ninaA[+]/ninaA^E110V, Scer\GAL4^ninaE.PT

(Mendes et al., 2009)

Suppressed by

Statement

Reference

p53^UAS.cJa has increased cell death phenotype, suppressible by ninaA¹/ninaA[+]

(Mendes et al., 2009)

Scer\GAL4^ninaE.PT, p53^UAS.cJa has increased cell death phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^ninaE.PT

(Mendes et al., 2009)

p53^UAS.cJa has increased cell death phenotype, suppressible by ninaA[+]/ninaA^E110V

(Mendes et al., 2009)

p53^UAS.cJa has increased cell death phenotype, suppressible by ninaA¹⁹/ninaA[+]

(Mendes et al., 2009)

p53^UAS.cJa has increased cell death phenotype, suppressible by ninaA^G98D/ninaA[+]

(Mendes et al., 2009)

NOT suppressed by

Statement

Reference

p53^UAS.cJa has increased cell death phenotype, non-suppressible by ninaA[+]/ninaA^R120K

(Mendes et al., 2009)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

BacA\p35^GMR.PH, Scer\GAL4^ninaE.PT, p53^UAS.cJa has outer photoreceptor cell phenotype, non-enhanceable by ninaA[+]/ninaA^E110V, Scer\GAL4^ninaE.PT

(Mendes et al., 2009)

Suppressed by

Statement

Reference

p53^UAS.cJa has outer photoreceptor cell phenotype, suppressible by ninaA[+]/ninaA^E110V

(Mendes et al., 2009)

p53^UAS.cJa has outer photoreceptor cell phenotype, suppressible by ninaA¹⁹/ninaA[+]

(Mendes et al., 2009)

p53^UAS.cJa has outer photoreceptor cell phenotype, suppressible by ninaA^G98D/ninaA[+]

(Mendes et al., 2009)

p53^UAS.cJa has outer photoreceptor cell phenotype, suppressible by ninaA¹/ninaA[+]

(Mendes et al., 2009)

Scer\GAL4^ninaE.PT, p53^UAS.cJa has outer photoreceptor cell phenotype, suppressible by BacA\p35^GMR.PH, Scer\GAL4^ninaE.PT

(Mendes et al., 2009)

NOT suppressed by

Statement

Reference

p53^UAS.cJa has outer photoreceptor cell phenotype, non-suppressible by ninaA[+]/ninaA^R120K

(Mendes et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Loss of one genomic copy of ninaA, in a ninaA^E110V/+, ninaA¹⁹/+, ninaA^G98D/+ or ninaA¹/+ heterozygous background (but not in a ninaA^R120K/+ background), protects the outer photoreceptor cells from Scer\GAL4^ninaE.PT;p53^Scer\UAS.cJa-induced apoptosis.

(Mendes et al., 2009)

Two-thirds of animals expressing p53^Scer\UAS.cJa under the control of Scer\GAL4^Act5C.PI in a DLP^U26/DLP^U26 background die in early pupal stage and only 9% of them eclose.

(Bodai et al., 2007)

Xenogenetic Interactions

Statement

Reference

The presence of a BacA\p35^GMR.PH background suppresses the outer photoreceptor cell death associated with Scer\GAL4^ninaE.PT-induced expression of p53^Scer\UAS.cJa. BacA\p35^GMR.PH-mediated p53^Scer\UAS.cJa inhibition is not enhanced by a ninaA^E110V/+ background.

(Mendes et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

p53^Scer\UAS.cJa

p53^UAS.cJa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Jin

(Jin et al., 2000)

Secondary FlyBase IDs

References (3)

Report Sections

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