FlyBase Allele Report: Dmel\Top1[hs.2]

General Information

Symbol

Dmel\Top1^hs.2

Species

D. melanogaster

Name

FlyBase ID

FBal0117630

Feature type

allele

Associated gene

Dmel\Top1

Associated Insertion(s)

Carried in Construct

P{Hsp70-ctop1-2}

Key Links

Transgenic product class

cDNA

(Zhang et al., 2000)

wild_type

(Zhang et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhang et al., 2000)

wild_type

(Zhang et al., 2000)

Mutagen

in vitro construct

(Zhang et al., 2000)

Progenitor genotype

Carried in construct

P{Hsp70-ctop1-2}

Cytology

Description

A 5.2kb Top1 cDNA is driven by a Hsp70 promoter.

(Zhang et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Top1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Top1⁷⁷

(Zhang et al., 2000)

wild-type

(Zhang et al., 2000)

Phenotype Manifest In

dorsal appendage, with Top1⁷⁷

(Zhang et al., 2000)

follicle cell, with Top1⁷⁷

(Zhang et al., 2000)

germarium, with Top1⁷⁷

(Zhang et al., 2000)

mitotic cell cycle, with Top1⁷⁷

(Zhang et al., 2000)

mitotic cell cycle | embryonic stage 4

(Zhang et al., 2000)

mitotic cell cycle | embryonic stage 4, with Top1⁷⁷

(Zhang et al., 2000)

mitotic telophase | embryonic stage 4, with Top1⁷⁷

(Zhang et al., 2000)

nucleus | embryonic stage 4

(Zhang et al., 2000)

nucleus | embryonic stage 4, with Top1⁷⁷

(Zhang et al., 2000)

nurse cell & nucleus, with Top1⁷⁷

(Zhang et al., 2000)

nurse cell | ectopic, with Top1⁷⁷

(Zhang et al., 2000)

oocyte, with Top1⁷⁷

(Zhang et al., 2000)

oocyte | ectopic, with Top1⁷⁷

(Zhang et al., 2000)

Detailed Description

Statement

Reference

Low levels of Top1^hs.2 expression lead to a greatly reduced number of nuclei in early embryos. Nuclei differ in size and morphology. telophase nuclei are found in addition to interphase nuclei. Also nuclei with extra amount of DNA are observed. presumably arising from non-disjunction or fusions after abnormal nuclear divisions.

(Zhang et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Top1^hs.2 rescues Top1⁷⁷

(Zhang et al., 2000)

Comments

Top1^hs.2 completely rescues the lethality of Top1⁷⁷ on induction by heatshock. When heatshock is carried out for the first 7 days of development up to 100% of mutants are rescued. Heat treatment during days 4 to 7 results in 79% rescue. Even without heatshock some rescue (larvae develop, but with a smaller size) is seen.

(Zhang et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Top1^hs.2

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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