The first 40 amino acids of fng are replaced by sequence encoding a transmembrane domain (Tag:Golgi(hGALNT3)), which functions as a Golgi-retention signal. Expression is under the control of the UAS sequences and it is also tagged by Tag:MYC.
Carried in a plasmid and transiently transfected into SL2 cells.
Carried in a plasmid and transiently transfected into SL2 cells.