FlyBase Allele Report: Dmel\Prim2[Sfaf240]

General Information

Symbol

Dmel\Prim2^Sfaf240

Species

D. melanogaster

Name

FlyBase ID

FBal0120996

Feature type

allele

Associated gene

Dmel\Prim2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Chen et al., 2000)

Progenitor genotype

Cytology

Description

Deletion of 101bp deleting amino acid residues 432 to 464 and resulting in a frame shift.

(Chen et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:20,360,115..20,360,215 [-]

Comment:

Approximate location of a 101bp deletion that removes amino acids G432 to G464 and causes a frameshift (exact endpoints not specified).

(Chen et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | third instar larval stage (with Prim2^T2)

(Chen et al., 2000)

increased occurrence of cell division | third instar larval stage (with Prim2^T2)

(Chen et al., 2000)

lethal (with Prim2^j10B2Δ11)

(Chen et al., 2000)

lethal (with Prim2^j10B2Δ12)

(Chen et al., 2000)

lethal (with Prim2^ppl-1)

(Chen et al., 2000)

lethal | recessive

(Chen et al., 2000)

lethal - all die before end of pupal stage (with Prim2^T2)

(Chen et al., 2000)

some die during pupal stage (with Prim2^T2)

(Chen et al., 2000)

viable (with Prim2^j10B2Δ13)

(Chen et al., 2000)

viable (with Prim2^j10B2Δ14)

(Chen et al., 2000)

viable (with Prim2^j10B2Δ15)

(Chen et al., 2000)

visible (with Prim2^j10B2Δ13)

(Chen et al., 2000)

visible (with Prim2^j10B2Δ14)

(Chen et al., 2000)

visible (with Prim2^j10B2Δ15)

(Chen et al., 2000)

Phenotype Manifest In

eye | anterior (with Prim2^T2)

(Chen et al., 2000)

eye disc (with Prim2^T2)

(Chen et al., 2000)

mitotic cell cycle (with Prim2^T2)

(Chen et al., 2000)

ommatidium (with Prim2^j10B2Δ13)

(Chen et al., 2000)

ommatidium (with Prim2^j10B2Δ14)

(Chen et al., 2000)

ommatidium (with Prim2^j10B2Δ15)

(Chen et al., 2000)

Detailed Description

Statement

Reference

Shows a rough eye phenotype in transheterozygous combination with DNAprim^j10B2Δ13, DNAprim^j10B2Δ14 or DNAprim^j10B2Δ15. Patterning of the accessory cells (cone and pigment cells) appears normal in DNAprim^Sfaf240/DNAprim^j10B2Δ13 flies and the photoreceptor cells are normally arranged. Cell death is seen in the anterior of the eye in DNAprim^T2/DNAprim^Sfaf240 late-stage pupae. More cells are in S phase at a given time compared to wild type in DNAprim^T2/DNAprim^Sfaf240 third larval instar eye discs.

(Chen et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Prim2^Sfaf240/DNAprim[+] is a suppressor of visible | recessive phenotype of faf^FO8

(Chen et al., 2000)

NOT Suppressor of

Statement

Reference

Prim2^Sfaf240/DNAprim[+] is a non-suppressor of female sterile | recessive phenotype of faf^FO8

(Chen et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

Prim2^Sfaf240/DNAprim[+] is a suppressor of ommatidium phenotype of faf^FO8

(Chen et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Prim2^j10B2Δ11

(Chen et al., 2000)

Prim2^j10B2Δ12

(Chen et al., 2000)

Prim2^ppl-1

(Chen et al., 2000)

Prim2^J1

(Chen et al., 2000)

Prim2^L5

(Chen et al., 2000)

Prim2^T2

(Chen et al., 2000)

Prim2^j10B2

(Chen et al., 2000)

Partially rescued by

Prim2^Sfaf240/Prim2^j10B2 is partially rescued by Scer\GAL4^Act5C.PI/Prim2^UAS.cCa

(Chen et al., 2000)

Comments

Expression of DNAprim^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PI partially rescues DNAprim^j10B2/DNAprim^Sfaf240 flies; a few adult escapers are seen that die after 1 day.

(Chen et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a dominant suppressor of the faf^FO8 eye phenotype.

(Chen et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DNApol-α60^Sfaf240

DNAprim^Sfaf240

Prim2^Sfaf240

S(faf)240

(Chen et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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