An Hsp70 promoter drives expression of Trl tagged at the N-terminal end with a 700bp HindIII fragment encoding the DNA-binding domain of Ecol\lexA.
Expression of Trlhs.T:Ecol\lexA using heat shock has no affect on eye pigmentation in flies carrying wEcol\lexA.310, wEcol\lexA.110 or wEcol\lexA.BHL4.