An Hsp70 promoter fragment (a 457bp XbaI-XmnI 5' non-translated sequence) is inserted into the SacI-blunted site of plasmid pBacΔSac. This places the Hsp70 promoter sequences upstream of the putative Tni\piggyBac\T promoter.
Carried in plasmid phsp-pBac, injected into D.melanogaster embryos and used as a helper plasmid (providing transposase function) for Tni\piggyBac mediated transformation.
Carried in plasmid "pBhs/ΔSac". Tested for activity in embryos of D.melanogaster, A.aegypti and T.ni.
Carried in plasmid phsp-pBac, injected into D.melanogaster embryos and used as a helper plasmid (providing transposase function) for Tni\piggyBac mediated transformation.