Construct: Nucleotide substitution: G-260A. Nucleotide substitution: C-259A. Nucleotide substitution: G-258A. Nucleotide substitution: A-257C. Nucleotide substitution: T-256C. Nucleotide substitution: A-255C. Nucleotide substitution: CGCTAT to AAACCC at -289 to -279bp. Mutations are clustered between the ovo protein binding sites.