The FRT cassette has been excised, juxtaposing twi enhancer sequences and the coding sequences which consist of mutant Kr fused in frame to the C-terminal portion of h (amino acid residues 255 to 337).
abdominal segment 5
abdominal segment 6
abdominal segment 7
abdominal segment 8
Expression of h::KrΔPED.twi results in the fusion of the 5th to 8th abdominal segments.