FlyBase Allele Report: Dmel\ptc[N.UAS]

General Information

Symbol

Dmel\ptc^N.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0124963

Feature type

allele

Associated gene

Dmel\ptc

Associated Insertion(s)

Carried in Construct

P{UAS-ptc.N}

Key Links

Transgenic product class

inframe_deletion

(Johnson et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Johnson et al., 2000)

Mutagen

in vitro construct

(Johnson et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-ptc.N}

Cytology

Description

UASt sequences drove expression of an XhoI/FspI fragment of ptc.

(Johnson et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ptc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

first posterior wing cell, with Scer\GAL4^71B, ptc^C.UAS

(Johnson et al., 2000)

first posterior wing cell, with Scer\GAL4^71B, ptc^CΔ.UAS

(Johnson et al., 2000)

submarginal wing cell, with Scer\GAL4^71B, ptc^C.UAS

(Johnson et al., 2000)

submarginal wing cell, with Scer\GAL4^71B, ptc^CΔ.UAS

(Johnson et al., 2000)

wing, with Scer\GAL4^en-e16E, ptc^C.UAS

(Johnson et al., 2000)

wing, with Scer\GAL4^en-e16E, ptc^CΔ.UAS

(Johnson et al., 2000)

wing vein L3, with Scer\GAL4^71B, ptc^C.UAS

(Johnson et al., 2000)

wing vein L3, with Scer\GAL4^71B, ptc^CΔ.UAS

(Johnson et al., 2000)

wing vein L3, with Scer\GAL4^en-e16E, ptc^C.UAS

(Johnson et al., 2000)

wing vein L3, with Scer\GAL4^en-e16E, ptc^CΔ.UAS

(Johnson et al., 2000)

wing vein L4, with Scer\GAL4^71B, ptc^C.UAS

(Johnson et al., 2000)

wing vein L4, with Scer\GAL4^71B, ptc^CΔ.UAS

(Johnson et al., 2000)

wing vein L4, with Scer\GAL4^en-e16E, ptc^C.UAS

(Johnson et al., 2000)

wing vein L4, with Scer\GAL4^en-e16E, ptc^CΔ.UAS

(Johnson et al., 2000)

Detailed Description

Statement

Reference

Expression of ptc^N.Scer\UAS when driven by Scer\GAL4^71B, or Scer\GAL4^69B causes no detectable wing phenotype. Expression of both ptc^N.Scer\UAS and either ptc^C.Scer\UAS or ptc^CΔ.Scer\UAS when driven by Scer\GAL4^71B causes both mild and severe wing phenotypes in the wing - partial fusion of wing veins 3 and 4 to the deletion of the region between veins 2 and 4. Expression of both ptc^N.Scer\UAS and either ptc^C.Scer\UAS or ptc^CΔ.Scer\UAS when driven by Scer\GAL4^en-e16E leads to a drastic loss of central wing pattern.

(Johnson et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

ptc^N.UAS/Scer\GAL4^da.G32 partially rescues ptc^CΔ.UAS

(Johnson et al., 2000)

Comments

(with ptc^CΔ.Scer\UAS) Fails to rescue: ptc¹⁶ { Scer\GAL4^71B } The addition of both ptc^N.Scer\UAS and ptc^C.Scer\UAS (when driven by Scer\GAL4^da.G32) to ptc¹⁶ embryos complements the cuticle defects of these embryos - instead of 20% of embryos having a "ptc" phenotype, only 1% do. The addition of ptc^N.Scer\UAS to ptc^CΔ.Scer\UAS (both driven by Scer\GAL4^71B) suppresses the wing notching phenotype (with ptc^C.Scer\UAS) Partially rescues: ptc¹⁶

(Johnson et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells for co-immunoprecipitation experiments.

(Johnson et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ptc^N.Scer\UAS

ptc^N.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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