FlyBase Allele Report: Dmel\NKAIN[EP560]

General Information

Symbol

Dmel\NKAIN^EP560

Species

D. melanogaster

Name

FlyBase ID

FBal0131348

Feature type

allele

Associated gene

Dmel\NKAIN

Associated Insertion(s)

P{EP}NKAIN^EP560

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}NKAIN^EP560

Cytology

Description

P{EP}NKAIN^EP560 is inserted outside the open-reading frame of NKAIN.

(Gorokhova et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,881,591..24,881,591 [+]

(Gorokhova et al., 2007, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal grooming behavior | heat sensitive

(Gorokhova et al., 2007)

lethal (with Df(2R)ED4071)

(Gorokhova et al., 2007)

paralytic | heat sensitive

(Gorokhova et al., 2007)

paralytic | heat sensitive (with Df(2R)ED4071)

(Gorokhova et al., 2007)

uncoordinated | heat sensitive

(Gorokhova et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

At room temperature, NKAIN^EP560 homozygous flies exhibit increased wing beating and excessive grooming compared with wild-type flies. When placed at 38[o]C, homozygous NKAIN^EP560 flies became severely uncoordinated and exhibit seizure-like movements and become paralyzed within 10 minutes. In contrast, NKAIN^EP560 heterozygous flies do not show such changes in behavior. When shifted back to room temperature, homozygous flies return to normal within 10-15min.

NKAIN^EP560/Df(2R)ED4071 flies are not viable. Only a very small number of escapers are found and they die shortly after eclosion. These escapers exhibit temperature-sensitivity paralysis when tested at 38[o]C.

(Gorokhova et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG34413^EP560

CG9047^EP560

NKAIN^EP560

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close