Flies expressing Atpα^D369N.hs show increased sensitivity to ouabain in a feeding assay compared to wild-type flies. They show bang sensitive paralysis and reduced open-field locomotor activity. Atpα²²⁰⁶ homozygotes that are also expressing Atpα^D369N.hs are more sensitive to ouabain in a feeding assay than Atpα²²⁰⁶ homozygotes which do not carry Atpα^D369N.hs. Atpα²²⁰⁶ homozygotes that are also expressing Atpα^D369N.hs take longer to recover following paralysis (induced by vigorous mechanical stimulation) than Atpα²²⁰⁶ homozygotes which do not carry Atpα^D369N.hs. Atpα²²⁰⁶ homozygotes are less active than wild-type flies in a reactive climbing assay. This defect is more severe if the flies are also expressing Atpα^D369N.hs.