FB2025_01 , released February 20, 2025
Allele: Dmel\out4
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General Information
Symbol
Dmel\out4
Species
D. melanogaster
Name
FlyBase ID
FBal0141001
Feature type
allele
Associated gene
Dmel\out
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Coffman et al., 2002)
Progenitor genotype
Cytology
Description

Mutation in a splice donor site.

(Yamada et al., 2008)

Nucleotide substitution: G?A.

(Yamada et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Programmed cell death of primordial germ cells (PGCs), which is essentially complete by stage 12 of embryogenesis in wild type, is disrupted in mutant embryos; the total average number of PGCs is normal in the mutants at stage 10, but although there is a gradual reduction in the number of PGCs, it is decreased compared to wild type, such that at stage 14 the average number of PGCs is higher in the mutant embryos compared to wild type.

      Although PGCs are able to incorporate into the gonad in mutant embryos, many germ cells ectopic to the gonad are seen at stage 14.

      (Yamada et al., 2008)

      Homozygous and hemizygous embryos show defects in germ cell development; many cells expressing a germ cell marker are seen outside the gonad, persisting until at least 15 hours of development. Mutant embryos appear to have normal segmentation.

      (Coffman et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      It has not been determined whether the lethality associated with the out4 chromosome is due to the out4 mutation or a second mutation present on the chromosome.

      (Coffman et al., 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      out4
      (Yamada et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)