FB2025_01 , released February 20, 2025
Allele: Dmel\out4
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General Information
Symbol
Dmel\out4
Species
D. melanogaster
Name
FlyBase ID
FBal0141001
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Progenitor genotype
Cytology
Description

Mutation in a splice donor site.

Nucleotide substitution: G?A.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Programmed cell death of primordial germ cells (PGCs), which is essentially complete by stage 12 of embryogenesis in wild type, is disrupted in mutant embryos; the total average number of PGCs is normal in the mutants at stage 10, but although there is a gradual reduction in the number of PGCs, it is decreased compared to wild type, such that at stage 14 the average number of PGCs is higher in the mutant embryos compared to wild type.

Although PGCs are able to incorporate into the gonad in mutant embryos, many germ cells ectopic to the gonad are seen at stage 14.

Homozygous and hemizygous embryos show defects in germ cell development; many cells expressing a germ cell marker are seen outside the gonad, persisting until at least 15 hours of development. Mutant embryos appear to have normal segmentation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

It has not been determined whether the lethality associated with the out4 chromosome is due to the out4 mutation or a second mutation present on the chromosome.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)