UAS regulatory sequences drive expression of 200bp antisense and sense nec fragments which are separated by a stable hairpin stemloop structure from the CopT replication control element.
Expression of necdsRNA.CopT.Scer\UAS under the control of Scer\GAL4Act5C.PI does not give a nec mutant phenotype. Expression of necdsRNA.CopT.Scer\UAS under the control of Scer\GAL4hs.PB using heat shock does not give a nec mutant phenotype.