Large mutant follicle cell clones are recovered at a low frequency compared to that of sibling twin spot clones. The cells often fail to form a coherent single-layered epithelium. Smaller mutant follicle cells clones usually show normal epithelial organisation, although occasionally single mutant cells fail to span the epithelium or more than one layer of cells is formed. Mutant follicle cells show an increase in the density of microtubules compared to their wild-type neighbours in mosaic egg chambers, when the egg chambers are fixed using an optimised procedure for preserving microtubules. When the egg chambers are fixed using a standard procedure, the mutant follicle cells appear to contain almost no microtubules, suggesting that the microtubules in the mutant cells are extremely unstable.

The number of pole cells is significantly reduced in most mutant embryos.