FlyBase Allele Report: Mmus\Ppp3ca[Act.UAS]

General Information

Symbol

Mmus\Ppp3ca^Act.UAS

Species

M. musculus

Name

FlyBase ID

FBal0144735

Feature type

allele

Associated gene

Mmus\Ppp3ca

Associated Insertion(s)

Carried in Construct

P{UAS-Mmus\Ppp3ca.act}

Transgenic product class

inframe_deletion

(Gajewski et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Gajewski et al., 2003)

Mutagen

in vitro construct

(Gajewski et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Mmus\Ppp3ca.act}

Cytology

Description

Expression of amino acids 1-398 of Mmus\Ppp3ca is under the control of UASt sequences. This truncation deletes the autoinhibitory and calmodulin-binding domains, making the phosphatase activity constitutive.

(Gajewski et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Ppp3ca

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^how-24B

(Gajewski et al., 2003)

some die during pupal stage, with Scer\GAL4^how-24B

(Gajewski et al., 2003)

Phenotype Manifest In

eye | P-stage, with Scer\GAL4^how-24B

(Gajewski et al., 2003)

pupal cuticle, with Scer\GAL4^how-24B

(Gajewski et al., 2003)

pupal epidermis, with Scer\GAL4^how-24B

(Gajewski et al., 2003)

pupal thorax, with Scer\GAL4^how-24B

(Gajewski et al., 2003)

Detailed Description

Statement

Reference

At the beginning of pupariation about 25% of Mmus\Ppp3ca^Act.Scer\UAS; Mmus\Ppp3ca^Act.Scer\UAS animals fail to completely evert their anterior spiracles and retain an elongated and sometimes curved larval shape, with abdominal contraction away from the pupal case. Most other animals initiate prepupal or pupal development, with the latter everting their head sacs and evaginating wing and leg discs during day 1. However, these animals often show a cleft thorax phenotype because of an incomplete fusion of the epidermis. Externally, these animals fail to progress in developmental terms by day 4 or later as no eye, cuticle, or bristle pigmentation is observed.

(Gajewski et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mmus\Ppp3ca^Act.UAS, Scer\GAL4^how-24B has lethal | pupal stage phenotype, suppressible by CanB2^EP774/CanB2[+], Scer\GAL4^how-24B

(Gajewski et al., 2003)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

CanB2^EP774/+ can recue the pupal lethality of Mmus\Ppp3ca^Act.Scer\UAS; Mmus\Ppp3ca^Act.Scer\UAS animals, resulting in the emergence of adults that are able to fly.

(Gajewski et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mmus\Ppp3ca^Act.Scer\UAS

Mmus\Ppp3ca^Act.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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