FB2026_02 , released June 18, 2026
Allele: Dmel\smoM2.UAS.GFP
Open Close
General Information
Symbol
Dmel\smoM2.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0146787
Feature type
allele
Associated gene
Dmel\smo
Associated Insertion(s)
Carried in Construct
P{UAS-smo.M2.GFP}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Zhu et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Zhu et al., 2003)
Mutagen
in vitro construct
(Zhu et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-smo.M2.GFP}
Cytology
Description

UASt regulatory sequences drive expression of smo containing the amino acid replacement W553L (the equivalent mutation in the human ortholog results in an oncogenic protein that is associated with basal cell carcinoma). In addition, a GFP tag has been inserted after the signal peptide sequence (after residue Ser 33).

(Zhu et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
smo
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:279,992..279,992 [+]
Nucleotide change:

G279992T

Amino acid change:

W553L | smo-PA

Reported amino acid change:

W553L

Comment:

Analogous W535L mutation in human SMO implicated in basal cell carcinoma; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Zhu et al., 2003)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Zhu et al., 2003)
      SMO:p.Trp535Leu
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: SMO_8117
      Comments concerning this variant
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      smoM2.Scer\UAS.T:Avic\GFP
      smoM2.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (4)